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novisyatria · 01-03-2010, 07:57 PM

Hi Frankco
yes you can use the crown as explant. its very good as explant source. take the shoot (part tip crown). for sterization using bleach 10 % ( 10 ml bleach + 90 ml sterile water) for 10 minute, rinse 5 x with sterile water, and then soak in alkohol 70 % for 3 minute. rise 5 5x with water. and explant ready to planted. try media MS + 2 mg BAP + 5 mg NAA for shoot multification.
good luck Frank. dont forget post your progress
regards

01-03-2010, 07:57 PM	#3 (permalink)
novisyatria Tissue Culturist novisyatria's Photo Gallery Location: Jakarta, Indonesia Name: Novi Syatria Join Date: Sep 2009 Posts: 480 BananaBucks : 421,147 Feedback: 0 / 0% Said "Thanks" 15 Times Was Thanked 697 Times in 205 Posts Said "Welcome to Bananas" 0 Times	Re: Tissue Culture Pineapple Hi Frankco yes you can use the crown as explant. its very good as explant source. take the shoot (part tip crown). for sterization using bleach 10 % ( 10 ml bleach + 90 ml sterile water) for 10 minute, rinse 5 x with sterile water, and then soak in alkohol 70 % for 3 minute. rise 5 5x with water. and explant ready to planted. try media MS + 2 mg BAP + 5 mg NAA for shoot multification. good luck Frank. dont forget post your progress regards __________________ Novi Syatria Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com