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Old 06-25-2007, 03:48 PM   #4 (permalink)
kgbenson
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Location: Columbia SC
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Name: Keith
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Default Re: SHoot-tip culture

Quote:
Originally Posted by mrbungalow View Post
Excellent page, very informative

But I have one question: After you use Phtotechs Multiplication medium for initiating the culture, wich one do you use for rooting? I don't think Phytotech sells a medium without the cytokinin?
I would go with w/either m519 (adding 2 gm/L gellan gum and 30 gm/L sucrose)or L467

Quote:
Phytotechlab really confuse me: Does the banana-multiplication medium really contain EVERYTHING (just add water as you say), or do you need to add more hormones etc.?
Yes. Just use as is. If you want to add more BAP, say up to 10 mg/L you can, but I wouldn't bother.

Quote:
And what then, is that other product called "banana basal-medium" for?
It is just another formulah, but from what I read, not as popular as the one from Vuylsteke.

Keith
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Last edited by kgbenson : 06-26-2007 at 12:45 PM.
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