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Old 06-25-2007, 09:26 PM   #6 (permalink)
kgbenson
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Location: Columbia SC
Zone: 8
Name: Keith
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Default Re: SHoot-tip culture

Quote:
Originally Posted by mrbungalow View Post
Excellent info Keith! Can't wait for your page to be finished.
I know, I know, heck it has taken me years to get this far . . .

Quote:
I failed miserably with my TC-trials this winter, but I suspect the explants were too small, and too weak. (Basically seedlings that have been sitting in the dark). I will have plenty of strong suckers in August-september to try.
Use larger source materials. The greater the distance from the outside to the meristem, the less the contamination - as a rule. And harvest the suckers shortly before you use them, sitting around invited contamination.

Quote:
Would you say some species are easier to TC than others?
Yes, and different groups behave differently. Plantains tend to form cormlets, where dessert bananas tend to form shoots. Some are very slow, others proliferate with abandon.

Keith
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