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jmoore · 08-18-2011, 07:40 AM

I'm not sure you could use UV light to sterilise media or explant.

The media vessels will absorb a large amount of UV light rendering it useless and it's penetrative power means you only sterilise the surface of the explant.

You would need to use gamma radiation which has many down sides not least of which it would damage the DNA of your explant.

08-18-2011, 07:40 AM	#2 (permalink)
jmoore jmoore 's Photo Gallery Location: Lowestoft, UK Zone: 8a Name: James Join Date: Aug 2008 Posts: 766 BananaBucks : 121,767 Feedback: 4 / 100% Said "Thanks" 192 Times Was Thanked 799 Times in 373 Posts Said "Welcome to Bananas" 71 Times	Re: 2 TC questions I'm not sure you could use UV light to sterilise media or explant. The media vessels will absorb a large amount of UV light rendering it useless and it's penetrative power means you only sterilise the surface of the explant. You would need to use gamma radiation which has many down sides not least of which it would damage the DNA of your explant.