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Old 09-01-2012, 10:56 AM   #8 (permalink)
sddarkman619
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Default Re: !st time Tissue Culturing with pictures

Well my third go at TC, and I have contamination in ALMOST all my jars, but a few are clean still. Did as most people say, soak in 10% bleach solution 10-15 minutes before placing in hood and rinsing with sterile water. Also not getting growth either. Using the banana tissue culturing medium from phytotech.
At least in my first attempt I was getting some growth, see here:
Some questions: Psuedostem, Meristem, where?

maybe someone could answer the question I posted in that post as well.

Here's the contamination I'm getting:


even though I 'm using a hood I use for mushroom cultivation, I'm still getting contams even though I am soaking the TC's and then slicing away the outer layers after rinsing in sterilized water.

I did however get some green going on this male flower :


this jar has no contams, but has some green going on.
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