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Gabe15 · 01-01-2008, 11:33 AM

Iʻm thinking about setting up little TC operation at my house next summer. I have some ideas about sterility, but I think with enough caution it might be possible. The biggest challenge I see is getting the media sterile prior to culturing, normally you would autoclave it, but there is that product called PPM (Plant Preservative Mixture) that is supposed to help, Ive tried it before without success but I think there was too much contamination from other things, if you made sure the vessels, tools and tissue was sterile, perhaps the PPM might work better if only the media needed sterilization.

01-01-2008, 11:33 AM	#16 (permalink)
Gabe15 Moderator Gabe15's Photo Gallery Location: Oahu, Hawaii Find me on the map! Zone: 12 Name: Gabe Join Date: Jul 2005 Posts: 3,892 BananaBucks : 13,351,057 Feedback: 5 / 100% Said "Thanks" 1 Times Was Thanked 8,241 Times in 2,200 Posts Said "Welcome to Bananas" 8 Times	Re: growing from seed or from TC? Iʻm thinking about setting up little TC operation at my house next summer. I have some ideas about sterility, but I think with enough caution it might be possible. The biggest challenge I see is getting the media sterile prior to culturing, normally you would autoclave it, but there is that product called PPM (Plant Preservative Mixture) that is supposed to help, Ive tried it before without success but I think there was too much contamination from other things, if you made sure the vessels, tools and tissue was sterile, perhaps the PPM might work better if only the media needed sterilization. __________________ Growing bananas in Colorado, Washington, Hawaii since 2004. Commercial banana farmer, 200+ varieties.