Re: Tissue Culture
Quote:
Originally Posted by druss
With regards to using the adult corm for tc, how long does the meristem last for after you cut it from the corm? Is it hrs? Days, can you preserve it for later use?
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There would be no reason to do this and really no way to. When you excise the tissue piece containing meristem, it needs to be put into culture immediately. You would only do this in a flow hood with everything set up and ready to go. There is maybe 5-10min of prep outside the hood, and then another 5-10min in the hood for initiation, but there should be no waiting on anything as this is the most critical time to avoid contamination and to get it right. If the initial culture is contaminated then nothing can be propagated from it. If you want to preserve meristem essentially, you either leave the plant intact and growing normally, or you put it into tissue culture, for all practical purposes there is no in-between.
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Growing bananas in Colorado, Washington, Hawaii since 2004. Commercial banana farmer, 200+ varieties.
Last edited by Gabe15 : 03-24-2017 at 10:32 AM.
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