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mrbungalow · 03-03-2008, 09:15 AM

This weekend, I went out to an old lady in Trondheim and bought an almost new pressure cooker. Apart from cooking lamb the old fashioned way, I am extremely happy to now have a reliable sterilizer for banana tissue culture!

I think this is what made me fail before, - unsanitary lab-conditions.

Beeing a very simple minded guy, I am going to try 2 approaches:

1. Germinating seeds and chopping the shoots off. Sterilizing these, make incisions, and putting them in multiplication media.

2. The "regular" shoot tip culture from a sucker.

Any ideas/ comments to this approach?

Erlend

03-03-2008, 09:15 AM	#1 (permalink)
mrbungalow Freezing member mrbungalow's Photo Gallery Location: Bergen, Norway Find me on the map! Zone: 8 Name: Erlend Join Date: Feb 2006 Posts: 598 BananaBucks : 52,366 Feedback: 0 / 0% Said "Thanks" 14 Times Was Thanked 165 Times in 78 Posts Said "Welcome to Bananas" 3 Times	Tissue Culture revisited This weekend, I went out to an old lady in Trondheim and bought an almost new pressure cooker. Apart from cooking lamb the old fashioned way, I am extremely happy to now have a reliable sterilizer for banana tissue culture! I think this is what made me fail before, - unsanitary lab-conditions. Beeing a very simple minded guy, I am going to try 2 approaches: 1. Germinating seeds and chopping the shoots off. Sterilizing these, make incisions, and putting them in multiplication media. 2. The "regular" shoot tip culture from a sucker. Any ideas/ comments to this approach? Erlend __________________