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Gabe15 · 03-04-2008, 03:19 AM

You don't need to sterilize your manipulation tools in the pressure cooker, I would recommend heating them over a flame just prior to use.

As for the tissue to use, I've been able to germinate embryos on the basic multiplication medium and it's not very hard to take out the embryos with a bit of practice. You may be able to put the seedlings directly into culture but I'm wondering how well they will be able to stand being submerged in bleach with such little tissue between the environment and the meristem, it's worth a try I suppose.

03-04-2008, 03:19 AM	#6 (permalink)
Gabe15 Moderator Gabe15's Photo Gallery Location: Oahu, Hawaii Find me on the map! Zone: 12 Name: Gabe Join Date: Jul 2005 Posts: 3,892 BananaBucks : 13,349,453 Feedback: 5 / 100% Said "Thanks" 1 Times Was Thanked 8,241 Times in 2,200 Posts Said "Welcome to Bananas" 8 Times	Re: Tissue Culture revisited You don't need to sterilize your manipulation tools in the pressure cooker, I would recommend heating them over a flame just prior to use. As for the tissue to use, I've been able to germinate embryos on the basic multiplication medium and it's not very hard to take out the embryos with a bit of practice. You may be able to put the seedlings directly into culture but I'm wondering how well they will be able to stand being submerged in bleach with such little tissue between the environment and the meristem, it's worth a try I suppose. __________________ Growing bananas in Colorado, Washington, Hawaii since 2004. Commercial banana farmer, 200+ varieties.