Gabe15

I sterilized the seeds in bleach for a few minutes, then cut them open on a sterile surface. The seeds are sterile on the inside if unplanted, I have had mixed results with seeds that have been in soil prior to culture, some showed instant contamination and others appeared to be clean (but have yet to germinate).

Alternatively, if you are working with fresh fruit, you can simply sterilize the fruit in bleach and then cut in open on a sterile surface and everything inside will be sterile, I have had good results with this and you also get the freshest embryos.

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Growing bananas in Colorado, Washington, Hawaii since 2004. Commercial banana farmer, 200+ varieties.