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Gabe15 · 11-10-2008, 04:51 AM

Some banana varieties are very slow in the normal media (such as Lakatan, Fe'i bananas, some Callimusa and Australimusa species), so to help them grow I use full strength MS (instead of the normal 1/2 strength), and its also been recommended to use 30g/L sucrose, currently we are using only 20g/L for normal micropropagation. There is also an issue with different varieties reacting adversely to BA, some varieties proliferate too much and go through some type of dedifferentiation to the point where I cannot regenerate usable explants from the tissue (perhaps it is possible with somatic embryogenesis, but that would be a whole different project...), so for these bananas that have reacted poorly to the BA (E. glaucum and Fe'i varieties), I will experiment with using little or no PGRs, especially upon initiation.

11-10-2008, 04:51 AM	#46 (permalink)
Gabe15 Moderator Gabe15's Photo Gallery Location: Oahu, Hawaii Find me on the map! Zone: 12 Name: Gabe Join Date: Jul 2005 Posts: 3,892 BananaBucks : 13,349,778 Feedback: 5 / 100% Said "Thanks" 1 Times Was Thanked 8,241 Times in 2,200 Posts Said "Welcome to Bananas" 8 Times	Re: Tissue Culture Some banana varieties are very slow in the normal media (such as Lakatan, Fe'i bananas, some Callimusa and Australimusa species), so to help them grow I use full strength MS (instead of the normal 1/2 strength), and its also been recommended to use 30g/L sucrose, currently we are using only 20g/L for normal micropropagation. There is also an issue with different varieties reacting adversely to BA, some varieties proliferate too much and go through some type of dedifferentiation to the point where I cannot regenerate usable explants from the tissue (perhaps it is possible with somatic embryogenesis, but that would be a whole different project...), so for these bananas that have reacted poorly to the BA (E. glaucum and Fe'i varieties), I will experiment with using little or no PGRs, especially upon initiation. __________________ Growing bananas in Colorado, Washington, Hawaii since 2004. Commercial banana farmer, 200+ varieties. Last edited by Gabe15 : 11-10-2008 at 12:29 PM.