The second trial is underway. I prepared the modified N&N medium with only 2 exceptions, first I added PPM and secondly, I used sucrose in place of maltose.
The biggest challenge I have is getting embryos out intact, those guys are tiny! The seeds I have are quite fresh, too, so it may be that the embryos are not fully mature. I have read something about embryo maturation. From what I recall, this explains why some of the older seeds actually germinate and fresh ones do not. This is contrary to what I understood, but I only scanned the article, so don't take this for gospel.
This medium is quite dark due to the activated charcoal used in preparation. I will post some photos in the gallery.
Keep your fingers crossed!
