Quote:
Originally Posted by griphuz
So is the idea that one embryo gives one plant, or do you want to devide the growint cells afterwards before producing plants?
And do you sterilise the embryo's before putting them in the culture?
Kind regards,
Remko.
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First we get the embryos growing, then they are divided once they start developing shoots. At that point, they are like normal TC plants.
I sterilize the seed surfaces first, then crack them open. The embryos are supposed to be sterile while inside the seed. I do as much of this under the hood as possible to minimize the chances of contamination. Sometimes you win, sometimes you lose.
I was not pleased with the first jar of embryos, so I did another set of them in another jar. This time I was pleased with the embryos I was able to extract. I do not recommend soaking them as this seems to make it more difficult to dissect the embryos. It seems easier to find them and get them out if the seeds are dry.