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Old 01-30-2009, 12:39 PM   #43 (permalink)
Chironex
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Location: Jakarta, Indonesia
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Default Re: Germination the easy way

Quote:
Originally Posted by griphuz View Post
If you have any info on that Scot, atricles of some kind. On the Silvernitrate sterilisation,...I would be very interested. Neder heared of people using that for sterilisation.
Kind regards,
Remko.
Remko, send a PM to me with your email and I will attache the research article in my reply. Being a heavy metal salt, special disposal laws may apply, so be cautious before you use it.

Quote:
Originally Posted by Gabe15 View Post
I have one E. perrieri beginning to grow on 1/2 strength MS with 1µl/L BA. It's too early to tell if its going to germinate normally or grow callus, I have had both happen depending on the species and medium. If they germinate fairly normally, then they are easy to deal with but if they grow callus then it can be hard to regenerate plants.
Gabe, that's great news. Was that 1 microliter/liter or 1 micromole/liter. Just being sure. I think a few of the surviving, uncontaminated E. perrieri are showing signs of growth, but not much!

I may take some photos of contaminated cultures to post so everyone can see what it looks like. Some of them are unique patterns.
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