Re: explant size?
As Gabe mentioned before, we generally use 1/2 strength M&S medium, with BA or BAP during initiation. I have been experimenting with other media too, including kinetin and activated charcoal. Although, these are primarily to promote embryo rescue.
Then no hormones for rooting, or some use NAA, IBA or IAA (auxins) but I haven't tried them yet, as all of my cultures are still in stage 2 sub-culturing.
__________________
Scot
|