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adrift · 08-12-2009, 08:17 PM

It doesn't matter what hormones or media you use if you have contamination. If the fungus or whatever is at the site of your meristem, then that was likely what brought the contamination. Examine your technique for processing it. If the fungus began away from the explant, then your general technique is bad.

If you have general overall contamination, you can practice without any explant or hormones. Make up your media and put in dishes. Keep them under the conditions you will be keeping your TCs for several weeks, watching for contamination. Don't bother making up jars / dishes with hormones and explants until you have your sterile technique worked out. Maybe under 10% contamination.

Did you use any preservative in the media (such as PPM)? Can you afford to do so?

Did you do it in open air or in a box? Can you make a box, even if it doesn't have airflow?

Have you practiced simply opening your jar or dish with one hand, keeping the lid over the top to avoid anything falling in, and putting the explant in with the other hand?

08-12-2009, 08:17 PM	#5 (permalink)
adrift adrift's Photo Gallery Location: Central FL Zone: 9a Name: KJ Join Date: Jan 2008 Posts: 174 BananaBucks : 17,763 Feedback: 2 / 100% Said "Thanks" 625 Times Was Thanked 197 Times in 87 Posts Said "Welcome to Bananas" 422 Times	Re: Tissue Culture MS Medium Help It doesn't matter what hormones or media you use if you have contamination. If the fungus or whatever is at the site of your meristem, then that was likely what brought the contamination. Examine your technique for processing it. If the fungus began away from the explant, then your general technique is bad. If you have general overall contamination, you can practice without any explant or hormones. Make up your media and put in dishes. Keep them under the conditions you will be keeping your TCs for several weeks, watching for contamination. Don't bother making up jars / dishes with hormones and explants until you have your sterile technique worked out. Maybe under 10% contamination. Did you use any preservative in the media (such as PPM)? Can you afford to do so? Did you do it in open air or in a box? Can you make a box, even if it doesn't have airflow? Have you practiced simply opening your jar or dish with one hand, keeping the lid over the top to avoid anything falling in, and putting the explant in with the other hand? __________________ I've got to stop asking people, "How stupid can you be." People are taking it as a challenge.