Medium MS Organic as Substitute Medium MS
sometime is hard to get or buy media MS for tissue culture. I want to share alternative media as substitute media MS. we called its Media MS organic. I hope this information will helpning you that interested with TC
Ingredient:
- Aquades 500 ml
- Coconut water 200 ml
- 4 gr fertilizer NPK 20 : 20 : 20 (like vitabloom, growmore, hyponex)
- Sugar 40 gr
- 7 gr seaweed (1 sachet)
- 500 gr bean spouts, mix in blender, filter (for rooting medium). For shoot medium change bean sprouts with stringbean
Methods
- Mix fertilizer, sugar, seaweed and bean sprout concentrate, coconut water in to baker glass ( 1 L). mix until homogenuos
- Add aquades until solution became 1 L
- Do PH 5,8
- Boiled the solution until boiling, put in the culture botol. Each bottle 25-30 ml solution. And autoclaving. wait medium until cold and ready for use
regards
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