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Welcome to the Bananas.org forums. You're currently viewing our message boards as a guest which gives you limited access to participate in discussions and access our other features such as our wiki and photo gallery. By joining our community, you'll have access to post topics, communicate privately with other members (PM), respond to polls, upload photos, and access many other special features. Registration is fast and simple, so please join our community today! If you have any problems with the registration process or your account login, please contact us. |
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10-18-2008, 10:17 AM
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#1 (permalink) |
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Join Date: Jun 2008
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New to the forum
Hello world.
I work at a tissue culture lab that does small fruit here in the US. I actually work in the greenhouse as Assistant Grower. we harden off the plants that come out of TC stage II media into plugs that are field planted. My true love for the lab stems from my love of fungi. After building my flowhood and learning sterile technique and agar.....i expanded my knowledge from microscopic tissue culture of spores and mycellium...into something slightly larger.....multicellular organisms. I found a local greenhouse that sold banana plants....that they tissue cultured. I purchased a few...Super Dwarf Cavendish, Rajapuri, and Velutina, with the hopes of enjoying a few fruit....maybe...... Now I feel I should do something with them....like TC! 2 plants are throwing pups. I plan on removing and cleaning them tomorrow. I'm going to search around the site....however....if someone has pictures of how large a pup has to be before removing for culture...THAT WOULD BE GREAT! I have NAA and 6-BA that I plan on using with MS media in ball jars. I order my chemicals through work...Sigma plant cell culture section. Well.....here I am. I'll post some pics of my hood at some point. |
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| The following 14 users say welcome MurCurY to Bananas.org! | ab2710 (03-09-2013), Abnshrek (06-27-2013), fruitguy@cox.net (04-22-2012), GoAngels (09-14-2010), harveyc (09-10-2010), Jezebel (09-12-2010), Kelso (05-01-2012), MediaHound (09-10-2010), PR-Giants (01-05-2013), scottu (10-23-2014), Scuba_Dave (07-09-2009), stumpy4700 (09-11-2010), sunfish (09-10-2010), Worm_Farmer (09-11-2010) |
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10-18-2008, 10:20 AM
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#2 (permalink) |
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Member
 
Location: Staten Island, NY - southernmost county in NY State.
Zone: USDA7- Sunset34
Join Date: Jun 2007
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Re: New to the forum
Looking forward to hearing more from you.
__________________
John 
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10-18-2008, 01:05 PM
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#3 (permalink) |
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Ensete nut
Location: Kuala Lumpur, Malaysia
Name: Tog
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Re: New to the forum
Welcome to the 'naner world. It will be nice to hear from your work on 'naner TC.
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10-18-2008, 02:00 PM
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#4 (permalink) |
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Tally-Man
 
Location: Florida
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Re: New to the forum
Welcome aboard!
__________________
Apologies in advance if I am slow to reply to your PM. I suggest posting in the forums for support if you need something urgent. |
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10-19-2008, 11:10 AM
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#5 (permalink) |
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3rd winter growing indoor
Location: Northern Indiana
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Re: New to the forum
Welcome aboard!
We are talking about trying TC at home!
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10-19-2008, 11:27 AM
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#6 (permalink) |
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The causasian Asian!
Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
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Re: New to the forum
Great to have you join us! Having experience in a TC lab is an added bonus. Those of us doing home TC benefit from your experience.
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Scot  |
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10-19-2008, 07:25 PM
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#7 (permalink) |
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More bananas less stress
Location: Savannah Georgia
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Re: New to the forum
Hello and welcome aboard.
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01-21-2009, 02:39 AM
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#8 (permalink) |
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The causasian Asian!
Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
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Re: New to the forum
The smallest pup that I have TC'ed so far was about 12-14" long. I was able to get the apical meristem quite easily. It is ready for its third sub-culture now.
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Scot |
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01-21-2009, 08:42 AM
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#9 (permalink) |
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banana junkie
Location: north carolina
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Re: New to the forum
welcome to the org....i havent tc anything...well at least not yet..i have my eyes on 2 types of nanas i would like to try putting together.....
__________________ WELCOME TO THE GATOR NATION  
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01-21-2009, 12:09 PM
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#10 (permalink) |
Location: Loves Park, Il.
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Re: New to the forum
Welcome to the Org.
__________________
 Bill  for my friend
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02-27-2009, 03:10 PM
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#11 (permalink) |
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Join Date: Jun 2008
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Re: New to the forum - (Tissue Culturing Banana Plants)
ok so....with things at work getting hectic i didn't get to the bananas when i wanted. I'm sick...so....tomorrow i'm going to disinfect and plate up a pup.
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02-27-2009, 04:28 PM
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#12 (permalink) |
Join Date: Mar 2008
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Re: New to the forum - (Tissue Culturing Banana Plants)
Welcome aboard!
 I look forward to you insights and experience!! :7)  |
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