How to care for tissue cultures or young plants.

pniksch · 03-22-2014, 06:29 PM

Who is the grower, and have you purchased from them before?
Glad to see they're hanging in there and thriving!
Thanks,
Paul

bananimal · 03-22-2014, 07:56 PM

Supplier is USDA ars - grin I believe. The PO made them what the are later on arrival. I can't get them - I'm not a commercial grower.

Abnshrek · 03-22-2014, 08:18 PM

Germplasm are much weaker than anything most people will order from WellSprings or the like.. Best thing is to keep them in semi-shade for 2 weeks and don't get them full sun till they are 18" or so for most of the south as it warms up or they will get fried especially come summer. :^)

PR-Giants · 03-22-2014, 10:39 PM

Quote:

Originally Posted by Abnshrek

Germplasm are much weaker than anything most people will order from WellSprings or the like.. Best thing is to keep them in semi-shade for 2 weeks and don't get them full sun till they are 18" or so for most of the south as it warms up or they will get fried especially come summer. :^)

In the Tropics germplasm are actually very strong, all of the plantlets survived and a few produced sister shoots. Being prepared and having confidence in your ability sure helps. The people who have problems are generally adding too much crap too early, like fish emulsion, azomite, mycorrhizae, and fertilizer. If spending more money will help the plant grow then simply buy a jewel encrusted Tiffany spray bottle.

I chose to set the sleeves so that the germplasm received full morning sun from the very first day and waited about 10 to 12 days before removing the sleeves completely. By hardening the plants more quickly they can grow faster by using the full sun.

This photo was posted on Feb-15, it is of a fully hardened Tigua

height 0.18".

Quote:

Originally Posted by PR-Giants

Super tall fully hardened TC

Abnshrek · 03-22-2014, 10:43 PM

That is a benefit of being in Banana Heaven.. :^)

ez · 03-22-2014, 10:54 PM

Quote:

Originally Posted by Abnshrek

Germplasm are much weaker than anything most people will order from WellSprings or the like.. Best thing is to keep them in semi-shade for 2 weeks and don't get them full sun till they are 18" or so for most of the south as it warms up or they will get fried especially come summer. :^)

It generally takes 4 to 5 weeks for plantlets that are removed from culture vessels to reach this size, after the initial hardening process (acclimatization) they will be transferred to a greenhouse.

They will need a secondary hardening process for two months in a greenhouse before they can be planted in the field.
__________________

Abnshrek · 03-22-2014, 11:03 PM

Quote:

Originally Posted by ez

It generally takes 4 to 5 weeks for plantlets that are removed from culture vessels to reach this size, after the initial hardening process (acclimatization) they will be transferred to a greenhouse.

They will need a secondary hardening process for two months in a greenhouse before they can be planted in the field.
__________________

That's about the size I've gotten from Well Springs garden, maybe slightly bigger but not much.. :^)

ez · 03-22-2014, 11:12 PM

Quote:

Originally Posted by Abnshrek

That's about the size I've gotten from Well Springs garden, maybe slightly bigger but not much.. :^)

Do they give you instructions on how to take care of them?

ez · 03-22-2014, 11:58 PM

Quote:

Originally Posted by Abnshrek

That's about the size I've gotten from Well Springs garden, maybe slightly bigger but not much.. :^)

Quote:

Originally Posted by ez

Do they give you instructions on how to take care of them?

Quote:

Originally Posted by PR-Giants

If you follow the instructions from the tissue culture lab, U. S. Department of Agriculture Tropical Agriculture Research Station, your plants are acclimatized in about 2 weeks. I probably saved the instruction sheet, but it matched perfectly to what I observed.

I meant instructions from wellspring gardens do they send you instructions with the plants.

I've read technical papers on the subject saying it would take three weeks or more.
Your technique of two weeks for hardening is impressive.

ez · 03-23-2014, 01:12 AM

Quote:

Originally Posted by PR-Giants

The most noticeable difference with your photo is that the plant is tall with small leaves,

and mine are short with large leaves, it would be nice to know the cultivar.

They don't say which cultivar

Preparing Tissue-Cultured Banana Plantlets for Field Planting - pdf

http://www.cialca.org/files/files/extension_materials/tissue-culture_english.pdf

Abnshrek · 03-23-2014, 11:41 AM

Quote:

Originally Posted by PR-Giants

Dan, u don't need no GRIN, u's got friends.

Shrek is sending u a Datil and I'll send u a Patupi the following year,

this way u can harvest both at the same time.

That's right.. Mine should be big enough to flourish this year.. :^)

@ Keith We know how you ( a professional banana grower grows), but you have to factor in people that have no experience growing banana's, and will just buy dirt thinking its all the same, which isn't so.. water crystals or water retainers in soil is one way to kill any banana quick up here, and certainly anywhere North of where I am.

Nicolas Naranja · 03-27-2014, 08:36 PM

I put the plants from GRIN underneath a bench that gets misted for about 2 weeks and then I place them on top of the bench.

For agristarts bananas, I put them in partial shade in a wind-protected location for 2 weeks and then move them out.

03-22-2014, 02:20 PM	#1 (permalink)
PR-Giants Commercial Grower Location: Florida & Greater Antilles Find me on the map! Join Date: Feb 2012 Posts: 4,616 BananaBucks : 4,254 Feedback: 16 / 100% Said "Thanks" 2,927 Times Was Thanked 12,649 Times in 3,693 Posts Said "Welcome to Bananas" 3,215 Times	How to care for tissue cultures or young plants. __________________ PR-Giants Photo Gallery 15,000+ Varieties Last edited by PR-Giants : 10-08-2015 at 06:03 PM.

03-22-2014, 06:29 PM	#2 (permalink)
pniksch Find me on the map! Join Date: Apr 2013 Posts: 60 BananaBucks : 6,418 Feedback: 0 / 0% Said "Thanks" 5 Times Was Thanked 46 Times in 29 Posts Said "Welcome to Bananas" 21 Times	Re: How to care for tissue cultures or young plants. Who is the grower, and have you purchased from them before? Glad to see they're hanging in there and thriving! Thanks, Paul

03-22-2014, 07:56 PM	#3 (permalink)
bananimal Member bananimal's Photo Gallery Location: Port St Lucie, Fla Find me on the map! Zone: 10a Name: Dan Join Date: Jun 2007 Posts: 2,512 BananaBucks : 570,957 Feedback: 12 / 100% Said "Thanks" 4,688 Times Was Thanked 4,979 Times in 1,509 Posts Said "Welcome to Bananas" 191 Times	Re: How to care for tissue cultures or young plants. Supplier is USDA ars - grin I believe. The PO made them what the are later on arrival. I can't get them - I'm not a commercial grower.

03-22-2014, 08:18 PM	#4 (permalink)
Abnshrek Happy Growing Abnshrek 's Photo Gallery Location: Beaumont Texas Find me on the map! Zone: 8b, but 9b weather.. Name: Migael / Michael Join Date: Aug 2009 Posts: 9,493 BananaBucks : 213,683 Feedback: 45 / 100% Said "Thanks" 10,447 Times Was Thanked 16,443 Times in 5,238 Posts Said "Welcome to Bananas" 2,318 Times	Re: How to care for tissue cultures or young plants. Germplasm are much weaker than anything most people will order from WellSprings or the like.. Best thing is to keep them in semi-shade for 2 weeks and don't get them full sun till they are 18" or so for most of the south as it warms up or they will get fried especially come summer. :^) __________________ I'm a Nannerhead :^)

03-22-2014, 10:43 PM	#6 (permalink)
Abnshrek Happy Growing Abnshrek 's Photo Gallery Location: Beaumont Texas Find me on the map! Zone: 8b, but 9b weather.. Name: Migael / Michael Join Date: Aug 2009 Posts: 9,493 BananaBucks : 213,683 Feedback: 45 / 100% Said "Thanks" 10,447 Times Was Thanked 16,443 Times in 5,238 Posts Said "Welcome to Bananas" 2,318 Times	Re: How to care for tissue cultures or young plants. That is a benefit of being in Banana Heaven.. :^) __________________ I'm a Nannerhead :^)

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03-27-2014, 08:36 PM	#13 (permalink)
Nicolas Naranja Muck bananas Nicolas Naranja 's Photo Gallery Location: Pahokee, FL Find me on the map! Zone: 10 Name: Nick Join Date: Nov 2008 Posts: 2,217 BananaBucks : 472,883 Feedback: 7 / 100% Said "Thanks" 66 Times Was Thanked 5,665 Times in 1,563 Posts Said "Welcome to Bananas" 7 Times	Re: How to care for tissue cultures or young plants. I put the plants from GRIN underneath a bench that gets misted for about 2 weeks and then I place them on top of the bench. For agristarts bananas, I put them in partial shade in a wind-protected location for 2 weeks and then move them out. __________________ Some people go bananas, I went plantains.

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