SHoot-tip culture

kgbenson · 06-24-2007, 09:37 PM

I have received a few emails/IMs requesting info about shoot tip culture of banana. I have re-done some of my original Banana Micro-propagation page. Feel free to visit it. It is still rough and only about 1/2 of the info is there. But there are some pics that answer the most common question I get, which is: what does the explant look like and how do I cut it out?

http://www.geocities.com/k2benson/TC...ban/TCban.html

I will be finishing it off over the next few weeks, fixing broken links etc.

Keith

D'Andra · 06-24-2007, 09:59 PM

Great info, thanx!

I'm trying to make a flow cabinet out of an old fridge. I'm not sure if I'm going to use vertical or horizontal flow though. My fear is that horizontal, blowing towards the front may cause uneven air flow because the filter is much smaller than the work area. Vertical flow just means more work to install but may give a more even pressure. Any ideas? Anyone? Anyone? Bueller? Bueller...?

mrbungalow · 06-25-2007, 12:31 AM

Excellent page, very informative!

But I have one question: After you use Phtotechs Multiplication medium for initiating the culture, wich one do you use for rooting? I don't think Phytotech sells a medium without the cytokinin?

Phytotechlab really confuse me: Does the banana-multiplication medium really contain EVERYTHING (just add water as you say), or do you need to add more hormones etc.? And what then, is that other product called "banana basal-medium" for?

Erlend

kgbenson · 06-25-2007, 03:48 PM

Quote:

Originally Posted by mrbungalow

Excellent page, very informative

But I have one question: After you use Phtotechs Multiplication medium for initiating the culture, wich one do you use for rooting? I don't think Phytotech sells a medium without the cytokinin?

I would go with w/either m519 (adding 2 gm/L gellan gum and 30 gm/L sucrose)or L467

Quote:

Phytotechlab really confuse me: Does the banana-multiplication medium really contain EVERYTHING (just add water as you say), or do you need to add more hormones etc.?

Yes. Just use as is. If you want to add more BAP, say up to 10 mg/L you can, but I wouldn't bother.

Quote:

And what then, is that other product called "banana basal-medium" for?

It is just another formulah, but from what I read, not as popular as the one from Vuylsteke.

Keith

mrbungalow · 06-25-2007, 03:54 PM

Excellent info Keith! Can't wait for your page to be finished.

I failed miserably with my TC-trials this winter, but I suspect the explants were too small, and too weak. (Basically seedlings that have been sitting in the dark). I will have plenty of strong suckers in August-september to try.

Would you say some species are easier to TC than others?

Erlend

kgbenson · 06-25-2007, 09:26 PM

Quote:

Originally Posted by mrbungalow

Excellent info Keith! Can't wait for your page to be finished.

I know, I know, heck it has taken me years to get this far . . .

Quote:

I failed miserably with my TC-trials this winter, but I suspect the explants were too small, and too weak. (Basically seedlings that have been sitting in the dark). I will have plenty of strong suckers in August-september to try.

Use larger source materials. The greater the distance from the outside to the meristem, the less the contamination - as a rule. And harvest the suckers shortly before you use them, sitting around invited contamination.

Quote:

Would you say some species are easier to TC than others?

Yes, and different groups behave differently. Plantains tend to form cormlets, where dessert bananas tend to form shoots. Some are very slow, others proliferate with abandon.

Keith

kgbenson · 06-25-2007, 09:28 PM

Quote:

Originally Posted by D' Andra

Great info, thanx!

I'm trying to make a flow cabinet out of an old fridge.

This is prolly way to large for what you need. Look into a smaller plastic hood and simplify the design.

Quote:

I'm not sure if I'm going to use vertical or horizontal flow though. My fear is that horizontal, blowing towards the front may cause uneven air flow because the filter is much smaller than the work area

.

A valid concern - but if the filter is sized to the chamber, it is a superior design.

Quote:

Vertical flow just means more work to install but may give a more even pressure. Any ideas? Anyone? Anyone? Bueller? Bueller...?

Resize the work area and go horizontal. JMHO

Keith

GATrops · 06-25-2007, 09:58 PM

Keith- Nice job on your pages and images!

kgbenson · 06-25-2007, 10:03 PM

Quote:

Originally Posted by GATrops

Keith- Nice job on your pages and images!

Thanks - there are several areas I really need to flesh out, particularly sterile technique. I think that trips up people in the beginning.

I also have a power point presentation I was supposed to give at the CRFG fruit festival several years ago. I couldn't make it as my mother was having some health issues. Ian McGuire gave the talk, but I still have the ppt if there is any way to post that on the web. (I am sure there is - but I haven't a clue)

Keith

MediaHound · 06-25-2007, 10:08 PM

If you hit reply to this thread, or when creating a new thread, etc..
There's a button that says "Manage Attachments".
Click that and then you can proceed to upload the ppt directly to your post for people to view on their computer.
Cheers

D'Andra · 06-26-2007, 06:22 PM

Thanx for your help Keith! My filter is 2'x2' so I guess that's what I'm building. Do you have any suggestions on the depth?
Again, I really want to thank you for your help & the link to your site.

mrbungalow · 06-27-2007, 02:20 AM

Keith, how often do you typically subculture a batch of banana-shoots? My aim would be to maintain a collection throughout the winter.

kgbenson · 06-27-2007, 06:57 AM

Quote:

Originally Posted by mrbungalow

Keith, how often do you typically subculture a batch of banana-shoots? My aim would be to maintain a collection throughout the winter.

Depending on the cultivar and the temps about ever 4-10 weeks, with every 6-8 being most common.

Keith

kgbenson · 06-27-2007, 01:46 PM

Quote:

Originally Posted by D' Andra

Thanx for your help Keith! My filter is 2'x2' so I guess that's what I'm building. Do you have any suggestions on the depth?

20 inches seems popular. I would think 18-24 would be good.

Look at this one: http://www.orchideenvermehrung.at/cg...sh/lfh/why.htm

Check out some of the mushroom growers forums. Those people have some pretty decent hood designs.

http://www.fungifun.org/English/Flowhood There are others.

Quote:

Again, I really want to thank you for your help & the link to your site.

My pleasure,

Keith

D'Andra · 06-29-2007, 08:57 PM

Thanx Keith!

Exactly the info I was looking for. Once again you've come through Big Time.
Let us know when you write a book. We get autographed copies, right?

In the morning I'm off to the Depot to get materials and supplies to build this.

No plant will be safe around me!

I can't wait for your site to be finished! I highly suggest anyone interested in MP look at the link in post #1.

kgbenson · 08-19-2007, 03:52 PM

Found a good, sheap text. I have had trouble locating it for people (and I won't give up my copy!)

http://www.bioversityinternational.o...asp?ID_PUB=248

Keith

06-24-2007, 09:37 PM	#1 (permalink)
kgbenson <div style="font-weight: bold;"><div style="font-weight: bold;">&lt;div style=&quot;font-weight: bold;&quot;&gt;&amp;lt;di v style=&amp;quot;font-weight: bold;&amp;quot;&amp;gt;&a mp;amp;lt;di v sty Location: Columbia SC Zone: 8 Name: Keith Join Date: Aug 2006 Posts: 132 BananaBucks : 41,644 Feedback: 1 / 100% Said "Thanks" 18 Times Was Thanked 47 Times in 31 Posts Said "Welcome to Bananas" 11 Times	Shoot-tip culture I have received a few emails/IMs requesting info about shoot tip culture of banana. I have re-done some of my original Banana Micro-propagation page. Feel free to visit it. It is still rough and only about 1/2 of the info is there. But there are some pics that answer the most common question I get, which is: what does the explant look like and how do I cut it out? http://www.geocities.com/k2benson/TC...ban/TCban.html I will be finishing it off over the next few weeks, fixing broken links etc. Keith __________________ Bee Sting Honey . . . So Good It Hurts http://www.geocities.com/k2benson/TC...ban/TCban.html Last edited by kgbenson : 08-19-2007 at 07:53 AM. Reason: spelling

06-24-2007, 09:59 PM	#2 (permalink)
D'Andra Senior Member D'Andra's Photo Gallery Location: Las Cruces, NM Find me on the map! Zone: 8 Join Date: Oct 2006 Posts: 224 BananaBucks : 4,329 Feedback: 0 / 0% Said "Thanks" 0 Times Was Thanked 9 Times in 7 Posts Said "Welcome to Bananas" 0 Times	Re: SHoot-tip culture Great info, thanx! I'm trying to make a flow cabinet out of an old fridge. I'm not sure if I'm going to use vertical or horizontal flow though. My fear is that horizontal, blowing towards the front may cause uneven air flow because the filter is much smaller than the work area. Vertical flow just means more work to install but may give a more even pressure. Any ideas? Anyone? Anyone? Bueller? Bueller...? __________________ D'.

06-25-2007, 12:31 AM	#3 (permalink)
mrbungalow Freezing member mrbungalow's Photo Gallery Location: Bergen, Norway Find me on the map! Zone: 8 Name: Erlend Join Date: Feb 2006 Posts: 598 BananaBucks : 51,565 Feedback: 0 / 0% Said "Thanks" 14 Times Was Thanked 165 Times in 78 Posts Said "Welcome to Bananas" 3 Times	Re: SHoot-tip culture Excellent page, very informative! But I have one question: After you use Phtotechs Multiplication medium for initiating the culture, wich one do you use for rooting? I don't think Phytotech sells a medium without the cytokinin? Phytotechlab really confuse me: Does the banana-multiplication medium really contain EVERYTHING (just add water as you say), or do you need to add more hormones etc.? And what then, is that other product called "banana basal-medium" for? Erlend __________________ Last edited by mrbungalow : 06-25-2007 at 03:16 AM.

06-25-2007, 03:54 PM	#5 (permalink)
mrbungalow Freezing member mrbungalow's Photo Gallery Location: Bergen, Norway Find me on the map! Zone: 8 Name: Erlend Join Date: Feb 2006 Posts: 598 BananaBucks : 51,565 Feedback: 0 / 0% Said "Thanks" 14 Times Was Thanked 165 Times in 78 Posts Said "Welcome to Bananas" 3 Times	Re: SHoot-tip culture Excellent info Keith! Can't wait for your page to be finished. I failed miserably with my TC-trials this winter, but I suspect the explants were too small, and too weak. (Basically seedlings that have been sitting in the dark). I will have plenty of strong suckers in August-september to try. Would you say some species are easier to TC than others? Erlend __________________

06-25-2007, 09:58 PM	#8 (permalink)
GATrops Member GATrops's Photo Gallery Join Date: Aug 2005 Posts: 153 BananaBucks : 62,312 Feedback: 0 / 0% Said "Thanks" 2 Times Was Thanked 42 Times in 17 Posts Said "Welcome to Bananas" 1 Times	Re: SHoot-tip culture Keith- Nice job on your pages and images!

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06-25-2007, 10:08 PM	#10 (permalink)
MediaHound Tally-Man MediaHound's Photo Gallery Location: Florida Find me on the map! Zone: 10 Name: Jarred Join Date: Jul 2005 Posts: 5,261 BananaBucks : 2,034,471 Feedback: 66 / 100% Said "Thanks" 3,856 Times Was Thanked 5,086 Times in 1,353 Posts Said "Welcome to Bananas" 2,086 Times	Re: SHoot-tip culture If you hit reply to this thread, or when creating a new thread, etc.. There's a button that says "Manage Attachments". Click that and then you can proceed to upload the ppt directly to your post for people to view on their computer. Cheers __________________ Apologies in advance if I am slow to reply to your PM. I suggest posting in the forums for support if you need something urgent.

06-26-2007, 06:22 PM	#11 (permalink)
D'Andra Senior Member D'Andra's Photo Gallery Location: Las Cruces, NM Find me on the map! Zone: 8 Join Date: Oct 2006 Posts: 224 BananaBucks : 4,329 Feedback: 0 / 0% Said "Thanks" 0 Times Was Thanked 9 Times in 7 Posts Said "Welcome to Bananas" 0 Times	Re: SHoot-tip culture Thanx for your help Keith! My filter is 2'x2' so I guess that's what I'm building. Do you have any suggestions on the depth? Again, I really want to thank you for your help & the link to your site. __________________ D'.

06-27-2007, 02:20 AM	#12 (permalink)
mrbungalow Freezing member mrbungalow's Photo Gallery Location: Bergen, Norway Find me on the map! Zone: 8 Name: Erlend Join Date: Feb 2006 Posts: 598 BananaBucks : 51,565 Feedback: 0 / 0% Said "Thanks" 14 Times Was Thanked 165 Times in 78 Posts Said "Welcome to Bananas" 3 Times	Re: SHoot-tip culture Keith, how often do you typically subculture a batch of banana-shoots? My aim would be to maintain a collection throughout the winter. __________________

06-29-2007, 08:57 PM	#15 (permalink)
D'Andra Senior Member D'Andra's Photo Gallery Location: Las Cruces, NM Find me on the map! Zone: 8 Join Date: Oct 2006 Posts: 224 BananaBucks : 4,329 Feedback: 0 / 0% Said "Thanks" 0 Times Was Thanked 9 Times in 7 Posts Said "Welcome to Bananas" 0 Times	Re: SHoot-tip culture Thanx Keith! Exactly the info I was looking for. Once again you've come through Big Time. Let us know when you write a book. We get autographed copies, right? In the morning I'm off to the Depot to get materials and supplies to build this. No plant will be safe around me! I can't wait for your site to be finished! I highly suggest anyone interested in MP look at the link in post #1. __________________ D'.

08-19-2007, 03:52 PM	#16 (permalink)
kgbenson <div style="font-weight: bold;"><div style="font-weight: bold;">&lt;div style=&quot;font-weight: bold;&quot;&gt;&amp;lt;di v style=&amp;quot;font-weight: bold;&amp;quot;&amp;gt;&a mp;amp;lt;di v sty Location: Columbia SC Zone: 8 Name: Keith Join Date: Aug 2006 Posts: 132 BananaBucks : 41,644 Feedback: 1 / 100% Said "Thanks" 18 Times Was Thanked 47 Times in 31 Posts Said "Welcome to Bananas" 11 Times	Re: SHoot-tip culture Found a good, sheap text. I have had trouble locating it for people (and I won't give up my copy!) http://www.bioversityinternational.o...asp?ID_PUB=248 Keith __________________ Bee Sting Honey . . . So Good It Hurts http://www.geocities.com/k2benson/TC...ban/TCban.html

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