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Main Banana Discussion This is where we discuss our banana collections; tips on growing bananas, tips on harvesting bananas, sharing our banana photos and stories.


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Old 03-28-2013, 01:34 PM   #1 (permalink)
 
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Default Temporary Immersion Systems

All,

I would love to hear from anyone who can share any ideas on simple designs for TIS systems. Also has anyone been trying any methods on how to derive protoplasts or embrygenic cell suspensions using a simple method that could be replicated in a home lab?

Ian
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Old 03-29-2013, 10:32 AM   #2 (permalink)
 
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Default Re: Temporary Immersion Systems

Please. What is a TIS?

George
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Old 03-29-2013, 03:01 PM   #3 (permalink)
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Default Re: Temporary Immersion Systems

Quote:
Originally Posted by George Webster View Post
Please. What is a TIS?

George
Temporary Immersion Systems

What is temporary immersion system and in what way this technique differ from other tissue culture techniques? - Yahoo! Answers
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Old 04-03-2013, 09:04 AM   #4 (permalink)
 
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Question Re: Temporary Immersion Systems

I have been able to find various types of TIS vessels (RITA, WAVE, UNDERTOW, BUBBLE BIOREACTORS etc.) However, I am wondering if there is anyone who has practical experiences to share with their operation, design and if there is any homemade type designs out there.
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Old 04-03-2013, 06:50 PM   #5 (permalink)
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Default Re: Temporary Immersion Systems

I remembered an excellent thread about this. I just found it and here is the link: Tissue Culture

Our member rohsen had posted several useful pdf's on this subject as well as others having to do with TC in general.
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