Banana Tissue Culture

malikopo39 · 06-19-2008, 11:41 AM

Hello all

I joined the Banana.Org Forum primarily for information on Bananas - growing bananas for a living. I have read a vast amount of information that is available on the web and would like to start my retirement enterprise from a scratch - that is from seed to a banana plantation.

I have been taken in by propagation of bananas by way of tissue culture and would like to try my hand at it but the laboratory-type regime does not suit the environment I am going to be operating in.

Firstly, at present there is no electricity but have a small generator and solar power to charge batteries, lights, etc.

I would like to hear from members who have had experience with tissue culture to help with information and suggestions on how this can be done in a rural environment in Zambia devoid of mod-cons.

I can set up a workable rudimentary laboratory to do the work required and get the hands-on experience to see if tissue culture would be a viable solution to propagating bananas that are disease-free.

A lot of work is being done in East Africa, West Africa but nothing is being done in Zambia although some commercial enterprises have been talking about it but is not viable proposition as yet.

Help will be much appreciated.

Kylie2x · 06-19-2008, 12:51 PM

Hey! A Big Welcome from Texas!!! It looks like you some very interesting work ahead of you!! I hope you will keep us updated on your progress!!! I can't answer your questions BUT I do look forward to seeing you around!!
Kylie

mskitty38583 · 06-19-2008, 12:55 PM

welcome! you have got a long road and a lot of work ahead of you. glad you joined us here at the org!!!

Chironex · 06-19-2008, 06:57 PM

Good luck with your plans. There are several threads here for tissue culture and I am sure you will find abundant information on these pages. Everyone here is eager to help you learn.

damaclese · 06-19-2008, 07:12 PM

welcome to Bananas.org. Gabe is are most educated member. hes talked at length regarding this subject. if you do a such I'm sure you will find at least something to get you started there are some vary good books on this topic but again Gabe would probably know better then i which periodicals and papers to direct you to.

mskitty38583 · 06-19-2008, 09:44 PM

Quote:

Originally Posted by damaclese

Gabe is are most educated member.

amen to that!!!!!

Richard · 06-20-2008, 07:18 PM

By the way ...

Quote:

Originally Posted by malikopo39

I joined the Banana.Org Forum primarily for information on Bananas - growing bananas for a living. I have read a vast amount of information that is available on the web and would like to start my retirement enterprise from a scratch - that is from seed to a banana plantation.

edible seedless bananas cannot be grown from seed ... they are seedless.

Chironex · 10-30-2008, 09:45 PM

Quote:

Originally Posted by malikopo39

Hello all

I joined the Banana.Org Forum primarily for information on Bananas - growing bananas for a living. I have read a vast amount of information that is available on the web and would like to start my retirement enterprise from a scratch - that is from seed to a banana plantation.

I have been taken in by propagation of bananas by way of tissue culture and would like to try my hand at it but the laboratory-type regime does not suit the environment I am going to be operating in.

Firstly, at present there is no electricity but have a small generator and solar power to charge batteries, lights, etc.

I would like to hear from members who have had experience with tissue culture to help with information and suggestions on how this can be done in a rural environment in Zambia devoid of mod-cons.

I can set up a workable rudimentary laboratory to do the work required and get the hands-on experience to see if tissue culture would be a viable solution to propagating bananas that are disease-free.

A lot of work is being done in East Africa, West Africa but nothing is being done in Zambia although some commercial enterprises have been talking about it but is not viable proposition as yet.

Help will be much appreciated.

You really don't need an elaborate laboratory. Tissue culture can be done in a cardboard box lined with foil or plastic. The main thing that you will need to do is to sterilize the equipment. 10-25% bleach and some 70% alcohol will help, but you will need a pressure cooker of some kind to be able to properly sterilize the jars, knives and most importantly the medium.
A small microwave oven could be used if you can get one. Boiling water won't kill all of the possible contaminants, so rely heavily on bleach water and alcohol.

Tog Tan · 10-31-2008, 01:09 AM

Welcome malikopo39, I sure admire your grit in your project. All the best!

chong · 10-31-2008, 01:41 AM

Quote:

Originally Posted by Chironex

...................................Boiling water won't kill all of the possible contaminants, so rely heavily on bleach water and alcohol.

Scot
I believe you. A lot of nurses and doctors use, and highly recommend alcohol for sterilization. So, I know that my insides are fairly sterile, what with the all the single malt scotch that I consume. Heavy, you say?? Don't know about bleach though, I don't use it myself. (LOL, I couldn't help this one!)

06-19-2008, 11:41 AM	#1 (permalink)
malikopo39 Join Date: Jun 2008 Posts: 1 BananaBucks : 11,835 Feedback: 0 / 0% Said "Thanks" 1 Times Was Thanked 0 Times in 0 Posts Said "Welcome to Bananas" 0 Times	Banana Tissue Culture Hello all I joined the Banana.Org Forum primarily for information on Bananas - growing bananas for a living. I have read a vast amount of information that is available on the web and would like to start my retirement enterprise from a scratch - that is from seed to a banana plantation. I have been taken in by propagation of bananas by way of tissue culture and would like to try my hand at it but the laboratory-type regime does not suit the environment I am going to be operating in. Firstly, at present there is no electricity but have a small generator and solar power to charge batteries, lights, etc. I would like to hear from members who have had experience with tissue culture to help with information and suggestions on how this can be done in a rural environment in Zambia devoid of mod-cons. I can set up a workable rudimentary laboratory to do the work required and get the hands-on experience to see if tissue culture would be a viable solution to propagating bananas that are disease-free. A lot of work is being done in East Africa, West Africa but nothing is being done in Zambia although some commercial enterprises have been talking about it but is not viable proposition as yet. Help will be much appreciated.

06-19-2008, 12:51 PM	#2 (permalink)
Kylie2x Senior Member Kylie2x's Photo Gallery Join Date: Sep 2006 Posts: 1,225 BananaBucks : 117,158 Feedback: 23 / 100% Said "Thanks" 313 Times Was Thanked 269 Times in 165 Posts Said "Welcome to Bananas" 160 Times	Re: Banana Tissue Culture Hey! A Big Welcome from Texas!!! It looks like you some very interesting work ahead of you!! I hope you will keep us updated on your progress!!! I can't answer your questions BUT I do look forward to seeing you around!! Kylie

06-19-2008, 12:55 PM	#3 (permalink)
mskitty38583 banana junkie mskitty38583 's Photo Gallery Location: north carolina Find me on the map! Zone: 7b Name: mskitty Join Date: Nov 2007 Posts: 4,071 BananaBucks : 195,040 Feedback: 26 / 100% Said "Thanks" 82 Times Was Thanked 890 Times in 617 Posts Said "Welcome to Bananas" 136 Times	Re: Banana Tissue Culture welcome! you have got a long road and a lot of work ahead of you. glad you joined us here at the org!!! __________________ WELCOME TO THE GATOR NATION

06-19-2008, 06:57 PM	#4 (permalink)
Chironex The causasian Asian! Chironex's Photo Gallery Location: Jakarta, Indonesia Find me on the map! Zone: I have no idea Name: Scot Join Date: Apr 2008 Posts: 2,788 BananaBucks : 122,590 Feedback: 9 / 100% Said "Thanks" 4,544 Times Was Thanked 1,406 Times in 808 Posts Said "Welcome to Bananas" 609 Times	Re: Banana Tissue Culture Good luck with your plans. There are several threads here for tissue culture and I am sure you will find abundant information on these pages. Everyone here is eager to help you learn. __________________ Scot

06-19-2008, 07:12 PM	#5 (permalink)
damaclese Living in Exile damaclese's Photo Gallery Location: Henderson NV Find me on the map! Zone: 9 Mediterranean climate Name: Paulo Join Date: Apr 2008 Posts: 1,767 BananaBucks : 288,898 Feedback: 0 / 0% Said "Thanks" 1,352 Times Was Thanked 1,620 Times in 706 Posts Said "Welcome to Bananas" 101 Times	Re: Banana Tissue Culture welcome to Bananas.org. Gabe is are most educated member. hes talked at length regarding this subject. if you do a such I'm sure you will find at least something to get you started there are some vary good books on this topic but again Gabe would probably know better then i which periodicals and papers to direct you to. __________________ Helping to foster understanding for the learning disabled

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10-31-2008, 01:09 AM	#9 (permalink)
Tog Tan Ensete nut Tog Tan's Photo Gallery Location: Kuala Lumpur, Malaysia Name: Tog Join Date: Jul 2008 Posts: 1,757 BananaBucks : 438,975 Feedback: 2 / 100% Said "Thanks" 1,011 Times Was Thanked 2,723 Times in 787 Posts Said "Welcome to Bananas" 204 Times	Re: Banana Tissue Culture Welcome malikopo39, I sure admire your grit in your project. All the best! __________________ ____ Tog Tan has sadly suddenly passed away 6/16/09. We will miss you Tog Tan.

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