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| Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside. |
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09-28-2009, 09:51 PM
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#1 (permalink) |
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Tissue Culturist
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Step by Step How To Tissue Culturing Bananas
step 1. Prepare the corm
step 2 scarep out skin (sorry i dont know right term) step 3 soak in bayklin (bleach) 25-30 minute and give it shake sometime step 4. rinse with sterile water 5 times step 5 put on sterile petri dish or plate. and reary to cut. prepare the tools before like pinset, scapel/knife, bunsen fire dll step 6. scarep out layer of stem has died (because bleach) aorund the explant step 7 cut vertically become 2 explant step 8 each explant put/planted in the culture bottle that has been prepared before. use MS media with 3-5 mg B enzyl amino purine (BAP)/liter solution step 9. after 2 weeks explant growth step 10 after 1 month explant ready for subculture
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Novi Syatria Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com |
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09-29-2009, 02:54 AM
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#2 (permalink) |
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Re: Step by Step How To Tissue Culturing Bananas
thanks...
You have done a great job in your lab. Those of us that are learning appreciate your posts aaron
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09-29-2009, 09:35 AM
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xmoose
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Re: Step by Step How To Tissue Culturing Bananas
This is Excellent information for people like me.
Thank you |
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09-29-2009, 02:10 PM
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#4 (permalink) |
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Got pink bananas?
 
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Re: Step by Step How To Tissue Culturing Bananas
Thanks for the pictures. I have always wondered how it was done.
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Austin Arkansas River Valley  Average January Low: 31°F, Average August High: 92°F Extreme Winter Lows: 5-15°F, Extreme Summer Highs: 100-112°F, Annual Precipitation: 52 inches |
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09-29-2009, 07:47 PM
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#5 (permalink) |
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Re: Step by Step How To Tissue Culturing Bananas
Could you post photos of the subculture process please. Thanks for the information.
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09-29-2009, 08:05 PM
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#6 (permalink) |
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Tissue Culturist
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Re: Step by Step How To Tissue Culturing Bananas
ok I will post soon.
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Novi Syatria Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com |
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09-29-2009, 09:01 PM
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#7 (permalink) |
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Re: Step by Step How To Tissue Culturing Bananas
Wow thanks! You saved me hours of reading mile long page after page...
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 A banana plant in the autumn gale - I listen to the dripping of rain Into a basin at night. ~ Basho Matsuo (1644- 1694)  
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09-29-2009, 09:16 PM
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#8 (permalink) |
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Tissue Culturist
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Re: Step by Step How To Tissue Culturing Bananas
dear blake
Yes if we reading textbook we needs much time. but what i post is very simple and practically. ihope you succes star with TC regards
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Novi Syatria Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com |
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09-29-2009, 09:27 PM
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#9 (permalink) |
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Re: Step by Step How To Tissue Culturing Bananas
Your very appreciated! ~JaNan
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09-29-2009, 10:24 PM
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#10 (permalink) |
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Re: Step by Step How To Tissue Culturing Bananas
Thanks so much for this thread! it is simple and helps me out alot
 Novi roxx! hehe!
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09-30-2009, 12:38 AM
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#11 (permalink) |
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Re: Step by Step How To Tissue Culturing Bananas
kool i hope some day to do it too.
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03-23-2012, 02:13 AM
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#12 (permalink) | |
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Re: Step by Step How To Tissue Culturing Bananas
Dear Novi,
Thank you for your step by step guide on TC of bananas. I am new in this forum with some basic knowledge of TC and was looking for help on doing TC of banana at home. Based on your step by step guide I have some questions:- 1. What is the ideal length and diameter of the suckers ? 2. Once I get the suckers (say from site of cultivation) , how long can they be stored at home before TC ? 3. I guess I may have to store it minimum 1 day at home before doing TC. How should I store them during this intermediate time ? 4. Is there any basic guidelines on preparing the crom ? Like how much to cut from the base and how much to cut from the top part (process between image 1 to image 2)? 5. What should be the ideal / average size of the prepared crom (step 1,image 2 )? 6. If I am doing TC of more than 1 sucker at one go (since I will prepare a min. 500ml or 1lit media), what is the sequence for sterilization ? Example :- If I have 10 suckers, do I need 10 different bottles of bleach and 10 different bottles of sterile water ? Or should I put all 10 into one large container containing bleach and another containg sterile water ? 7. Which part of the sterilization process can be done outside the hood and which part inside the hood ? 8. What should be the ideal / average size of explant before dividing into two (after step 6, image 6)? I am not clear regarding the above. Clarifications on the above doubts would be of great help. Thanks in advance. Regards, Amit Quote:
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04-10-2012, 08:35 PM
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#13 (permalink) | |
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Tissue Culturist
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Re: Step by Step How To Tissue Culturing Bananas
Quote:
1. suckers? you mean corm ? or plantlets? 2. we can keep corm as long as its still good about aweek 3. keep it at temperature room and dry 4. no yet 5. about 5-8 cm diameter 6. i have 2 corm in one bootle so more economic 7. stage 1-2 outside hood but mext must do in hood/LAminar 8. about 3 cm sorry my english not well. hope you succes with your TC
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04-22-2012, 10:05 PM
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#14 (permalink) |
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Re: Step by Step How To Tissue Culturing Bananas
I'm new here, and read everything I could here, but still don't see the actual step by step with explicit instructions and details. is this the place to find it ? or do I need to buy a manual......
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04-22-2012, 10:12 PM
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#15 (permalink) | |
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Re: Step by Step How To Tissue Culturing Bananas
Quote:
Simply Hydroponics Step by Step Tissue Culture part 1 | Household Hydroponics Last edited by sunfish : 04-22-2012 at 10:18 PM. |
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04-22-2012, 11:16 PM
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#16 (permalink) |
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Re: Step by Step How To Tissue Culturing Bananas
Good videos about plant cutting tissue culture, but not bananas
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04-22-2012, 11:20 PM
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#17 (permalink) | |
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Re: Step by Step How To Tissue Culturing Bananas
Quote:
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04-23-2012, 01:12 AM
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#18 (permalink) |
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Re: Step by Step How To Tissue Culturing Bananas
There is a book you can buy, it's called 'plants in a test tube' or something lik that, but again doesn't specifically cover bananas. You need to read the threads and a few scientific papers and kind of go from there. This section is full of excellent instructions on how to tissue culture bananas, you just have to look for it.
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04-23-2012, 06:52 AM
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#19 (permalink) |
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Re: Step by Step How To Tissue Culturing Bananas
Tissue culture techniques of Banana
https://docs.google.com/viewer?a=v&q...92RBi_mkW_wHXg http://www.academicjournals.org/ajb/...%20et%20al.pdf Last edited by sunfish : 04-23-2012 at 07:17 AM. |
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04-23-2012, 03:00 PM
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Re: Step by Step How To Tissue Culturing Bananas
Thanks, great thread!
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