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| Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside. |
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10-07-2009, 03:14 AM
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#1 (permalink) |
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Tissue Culturist
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Step by Step Subculture In Tissue CUlture Bananas (part 1)
Hi all
I promise you all to take photo subculture in TC bananas step by step. thats why i I'v done today. enjoy the pic. Step 1. Explant banana 1month old. ready for subculture 1 (Photo 1) Step 2. Put the explant on sterile petri dish/plate (Photo 2) Step 3. Cut vertically become 2 explant (Photo 3) Step 4. put the explant/planted in the bootle. media same MS + 5 BAP/l (Photo4) Step 5. After 1 month next, explant has grow and ready for subculture 2. explant will have few baby bananas. about4-7 baby (photo 5) Step 6. Put the explat on sterile petridish (Photo 6) Step 7. Cut the explant become few. every parts could be consist of 2-3 baby bananas (Photo 7) Step 8. put the explant/planted in the bootle. media same MS + 5 BAP/l (Photo8). Look at the next thread Next Step like subculture 1 or subculture 2
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Novi Syatria Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com |
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10-07-2009, 11:18 AM
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#2 (permalink) |
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I think with my banana ;)
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Re: Step by Step Subculture In Tissue CUlture Bananas (part 1)
A thread many of us have been waiting for. Thanks for this one, pal.
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Thnx to Marcel, Ante, Dr. Chiranjit Parmar and Francesco for the plants I've received.  Zeitgeist - Corporatocracy 101 (~2hrs) Zeitgeist - Moving Forward (~2.5hrs) |
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10-07-2009, 12:11 PM
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#3 (permalink) |
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Re: Step by Step Subculture In Tissue CUlture Bananas (part 1)
That's great!....Thanks for taking the time to share the information and photos.
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10-07-2009, 04:27 PM
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#4 (permalink) |
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Cold-tolerant collector
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Re: Step by Step Subculture In Tissue CUlture Bananas (part 1)
Very Nice!
Could you give us a little more detail though about each stage, and how you got there, and to the next stage? Kind regards, Remko. |
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10-07-2009, 07:20 PM
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#5 (permalink) |
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Tissue Culturist
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Re: Step by Step Subculture In Tissue CUlture Bananas (part 1)
Hi Remko
before this thread i hav been post step by step how TC bananas. just look in TIssue Culturing Forum jump regrads
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Novi Syatria Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com |
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10-07-2009, 08:41 PM
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#6 (permalink) |
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Re: Step by Step Subculture In Tissue CUlture Bananas (part 1)
sweet@!
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Brad |
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10-09-2009, 02:17 PM
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#7 (permalink) |
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xmoose
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Re: Step by Step Subculture In Tissue CUlture Bananas (part 1)
Awesome !!!
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04-24-2019, 10:04 PM
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#8 (permalink) |
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Re: Step by Step Subculture In Tissue CUlture Bananas (part 1)
I am facing problems in the shoot multiplication step.Can you please help me out why there is no growth even after adding adequate hormones and frequent subcultures?
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04-30-2019, 09:03 PM
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#9 (permalink) | |
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Tissue Culturist
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Re: Step by Step Subculture In Tissue CUlture Bananas (part 1)
Quote:
thanks
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Novi Syatria Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com |
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05-19-2020, 10:20 AM
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#10 (permalink) |
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Re: Step by Step Subculture In Tissue CUlture Bananas (part 1)
Nice photos, pretty remarkable this plant can be divided so early and often
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05-22-2021, 09:22 AM
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#11 (permalink) |
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Re: Step by Step Subculture In Tissue CUlture Bananas (part 1)
Did you figure out why no growth ? I am trying TC and same problem. Thanks
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04-15-2022, 02:43 PM
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#12 (permalink) |
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Re: Step by Step Subculture In Tissue CUlture Bananas (part 1)
thx. More details would be great.
What is "MS + 5 BAP/l ". Murashige and Skoog=ms? 6-benzyl aminopurine=bap? is it 5mg bap/Liter? is the bap dissolved into NaOH or another chemical? I am thinking, depending on the expense, in a few years trying to GE the coda gene to bananas [supposedly makes plants produce Glycinebetaine and be more cold hardy]. I have no background in GE, nor in growing banana's like this, and I can't stomach [ nor religiously] study biology at a university level. https://www.researchgate.net/publica...ences_Mediated ...if someone wants to beat me to it. |
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