novisyatria

last day i make media MA for tc from male flower bananas. and today will start new tc from male flower. its be my first time tc by using male flower. wish me luck. i 'll soon the pic about its process

regards

__________________
Novi Syatria
Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com

kamayoi

Wish u Good Luck Pak Novi..

hehe... hope u`ll show us step by step ..like how to identifyin male flower ..

AV1611Corbin777

Very cool, you should change your name to "TC mad man'
Any of these tc's your doing going to find their way over to the states?

__________________
The first law of thermodynamics, an expression of the principle of conservation of energy, states that energy can be transformed (changed from one form to another), but cannot be created or destroyed.
Source: http://en.wikipedia.org/wiki/Thermod...thermodynamics

novisyatria

today i did tc bananas from male flower and take photo for you all. enjoy it and wish me luck..

step 1. prepare the bananas flower. in this case i have musa velutina (no 1 and 2), new hybrid (3), ensente glucam (4),


Step 2 throw out the skin/layer of flower (what term?) until its only about3-4 cm length and soak in bleach 15 % for 10 minute and then rinse with sterile distilled water 5 times



step 3. open the each layer and take its male flower. best explant is flower in layer no 8 -16 from tip. flower no 1-7 and more than 16 is not good. its callus not embrionic. so cant to turn be shoot.


step 4 put the male flower in media MA. wait 1 month or more until they getting callus. and we can do subculture in same media to get more callus or put in liquid media if do tc cell suspension, or put in shoot induction media.


well, i will post pic later if there is good progress. hah.. i apologize my english is limited to explaining this thread. i hope you all understand what i mean. hah.. tc male flower more complicated than normal tc using the corm. must be carefull. its explant is so little

regards

__________________
Novi Syatria
Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com

xavierdlc61887

GREAT JOB!!! novi as always you you amaze me, Im glad i am learning from you!

kamayoi

good luck bro...
tq for great post..

novisyatria

well, after 9 days, tc male flower looks good so far and they looks star to growwth.
Banana Gallery - IMGP2883_tc_male_M_velutina
Banana Gallery - IMGP2884_tc_male_flower_m_velutina2

__________________
Novi Syatria
Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com

xavierdlc61887

Good job novi!!! i havent done anything too much work and no time to TC

novisyatria

After 1 month, tc male flower bananas has shows good progress. they forming callus/embrio. bothe ensente and m velutina should remove to new fresh media to get more embrio
Ensente Glaucum. they looks great


Musa Velutina also shows good response, even also producing a little browning


I am happy see them all growth

__________________
Novi Syatria
Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com

novisyatria

New pic Tc Male flower. they needs subculture soon

Ensente glaucum


M Velutina




regards

__________________
Novi Syatria
Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com

griphuz

Hi Novi,
Why do you use male flowerparts to TC? Does this give any other results then from meristematic tissue from a sucker?
And do you use other media/hormones to get this to work? Do you have some literature about it?

When you say "its callus not embrionic. so cant to turn be shoot." I think callus doesn't matter right? Can't you induce shootgrowth first, and then root growth from callus?

Good luck in the process, keep us updated! Thanks!

Kind regards,
Remko.

xavierdlc61887

griphuz, Hello

Novi has not been on for a while now due to some personal things going on at the moment, I will notify him about your question

xavierdlc61887

Ask Gabe, he will tell you what you want to know

Gabe15

It is possible to regenerate plants from the callus tissue, but it is not easy or simple. This technique is generally used to establish embryogenic cell suspensions used for somatic embryogenesis. Each somatic embryo develops from a single cell. This is generally only used when genetic engineering the plants so that the transformed plants (with new genetic material) are sure to have originated from a single cell, rather than having chimeras with some transformed and some non-transformed tissue, making it not only unstable, but hard to tell if something has been transformed or not. In Novi's case, I believe it is just for fun to see how far he could get using male flowers. To propagate plants via somatic embryogenesis is more trouble than it is worth for standard micropropagation, so it must just be for the fun of it.

__________________
Growing bananas in Colorado, Washington, Hawaii since 2004. Commercial banana farmer, 200+ varieties.

griphuz

Thanks again Gabe,
I'll try to get some literature of this matter, and read into it a little bit more.

Kind regards,
Remko.

novisyatria

yes, i do tc male flower for fun and only for ornamental bananas. so its okey if there is mutation or chemeras

__________________
Novi Syatria
Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com

sddarkman619

You said use only the flowers from layers 8-16, how do you know which are those layers? Slice it down the middle and count?
thanks

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