rohsen

hi there here's an article on using growth retardants for cultivating Musa in temporary immersion systems, people interested in using these systems can contact me if they please

ps i just found i had already posted this paper....???!!! sorry for that

Attached Images

Use of growth retardants for Musa iin TIS systems.pdf (1.15 MB, 115 views)

__________________
Reality Is What You Can Get Away With!!
Robert Anton Wilson

rohsen

jack draw, griphuz, chironex, after reading your discussion heres some reading material for you all

Attached Images

	ANALYZING SOMACLONAL VARIATION IN MICROPROPAGATED BANANAS (MUSA SPP.).pdf (84.7 KB, 69 views)
	Somatic embryogenesis versus zygotic embryogenesis in Ensete superbum.pdf (622.5 KB, 55 views)
	SOMATIC EMBRYOGENESIS AND PLANT REGENERATIONThrough cell suspensions in musa acuminata.pdf (1.04 MB, 57 views)
	Regeneration of banana (Musa spp. AAB cv. Dwarf Brazilian) via somatic embryogenesis.pdf (267.8 KB, 59 views)

Attached Files

	An improved protocol for microcallus production and whole plant regeneration from recalcitrant banan (327.1 KB, 55 views)
	Somatic embryogenesis of the banana hybrid cultivar FHIA-18 (AAAB) in liquid medium and scaled-up in (141.0 KB, 42 views)
	Inflorescence proliferation for somatic embryogenesis induction and suspension-derived plant regener (437.6 KB, 40 views)

__________________
Reality Is What You Can Get Away With!!
Robert Anton Wilson

rohsen

and 2 more publications

Attached Images

	REGENERATION OF PLANTS FROM ALGINATE-ENCAPSULATED SOMATIC EMBRYOS OF BANANA.pdf (384.0 KB, 88 views)
	Plant regeneration through direct somatic embryogenesis from protoplasts of banana.pdf (1.34 MB, 82 views)

__________________
Reality Is What You Can Get Away With!!
Robert Anton Wilson

rohsen

some clarification on these texts
alginate encapsulation is where you drop a somatic embryo in a sodium alginate solution thus creating what is known as a synthetic seed. wonderfull though complex technique.
Soma means "coming from the self" genesis "to give birth" so soma-tic embryo-genesis stands for creating embryos from the plants own cells, be they leaf, flower, stem or any other cell. (with different results )
protoplasts are basically cells with their cell walls removed allowing for "easy" in vitro crossbreeding and all sorts of other "easy" tricks (note the cynical """ marks around easy)All these techniques are pretty advanced. but it never harms to read ...right?

ps its always cool (for posting members) to ask me for specific articles, i have access to most scientific databases

__________________
Reality Is What You Can Get Away With!!
Robert Anton Wilson

alah

Hi, Keith. This is the kind of lecturing that I like, I need.. Please excuse me everybody else, you see, I´m areal novice. So keith, hope you´re still around and able to continue this with lecturing on the rest of the process.
Thanks, Alberto.

reenaevm

hi all,

had a query regarding multiplication of plantlets in vitro.
i have successfully initiated and got around 3-4 shoots from each initiated corm. these hav now grown into lil plantlets.

i want to know if it is possible to further take them for multiplication and how to go about with the multiplication. i kno i need to cut off all the leaves and roots but i'm confused about whether i have to discared the original corm tissue or not, the original corm has started blackening and some of the leaves have also started yellowing.

I'm a first timer with banana tc, so i'v really no idea how to go about it.
the variety i'm culturing is grand naine bananas

thanks in advance

reena

rohsen

as a rule i always reuse the parts that gave me shoots!! there may be many buds left in the part that already gave you some ... just cut of the browned bits and replant. nothing you can lose there...
regards dave

__________________
Reality Is What You Can Get Away With!!
Robert Anton Wilson

alah

Help. This article seems very useful to me, but when I try to download it I get an error message saying " Address not valid". Why, Alberto.

chong

Check your computer's security settings. That may be preventing your computer from downloading this file. Or, you may not have enough RAM or HD memory. I just downloaded it again, just now, and I was able to save it as a *.html file. Another way that I saved it, just now also, was to highlight the entire article, and then copy and paste it on to a Word document. The latter procedure assumes that you are able to open the link to the article.

Hope that works for you.

Chong

jmoore

http___www.geocities.com_k2benson_TCoverview_TCban_TCban.pdf here's a pdf of the same article

alah

Thanks, Chong. Something´s gone wrong with my CPU.
It hangsup every once in a while. I´ll check tomorrow.

alah

Thanks, James. I think I´ll stick to this protocol mainly because Phytotech has a distributor at Colombia. That means they´re right next to me and I can pay in our currency ( not US$ at 600% the official exchange!)

alah

Excuse me Keith. I received this email, with use of Phyto Tech Lab products for banana MP. I have some doubts with their explanation, as well as your´s. Can you please check and clarify thanks, Alberto.

Attached Files

Banana MP Facts.doc (243.0 KB, 93 views)

ligature

kindly let me know about automisation of micropropogation by using bioreactors
madhu

kamayoi

http://www.agriculture.gov.bn/DOA200...banana_TC3.pdf --> source : Brunei Agricultural research centre
banana TC..

ligature

thanks for your information kindly give some company reference who will install bioreactors for our commercial tissue culture lab in India.

ligature

can any body give more info about bio-reactors technology for micro-propagation for commercial tissue culture units and even the company which supply and install equipments.

B S MADHUSUDHAN
info@ligaturebiotechnologies.com
ligature@live.in
+91 9449837234

kamayoi

ur welcome fren..

dinhml

Hi guys, Here's my 1 day reading


it's not that difficult as you think it is. try it. it should be a fun and also a challenge. different authors have different formulas, but it all boils down to.


find all necessary utilities, P.H. meter, pressure cooker, micropipettes, 250ml flash, electronic scales, forceps


steriles all of these in pressure cookers @ 250F, or with strong ethanol or isopropyl alcohol

ingredients:

1 L of de-ionized water

4.3 gram of MS (Murashige and Skoog salts)

5ml of BAP, sometimes called BATH for nutrients purposes(studies show 5ml of BAP is the minimum to get the maximum of plantlets out of the test tubes or whatever containers)

1ml PPM(plant preserve material) for antimicrobial purpose (i think imma experimenting skipping this PPM step) - BUY HERE


AGAR, which is jelly that comes in patri dishes in most labs, which i think the powder form can be obtain thru the supermarkets for making jelly, which then u can boil with water and make AGAR out of it

Add Sugar and coconut milk for experimenting, since all formulas are self-made by a guy in his lab, so can we too . i suspect u add sugar in this step if u gonna mix the powder agar with water by yourself. Coconut milk acts as a growth promotor so add it if u have any.
add all the stuffs above together in a sterile flash/container and measure the P.H., try to have it 5.8, if PH too high, add vinegar, if too low, add antacid tablets. PH is crucial, the exacter to 5.8, the better


then boil in pressure cooker or microwave it until everything boil

let the mixture cool

pour in jars (where explant gonna go)

_____________________________
FOR the Corm/or Explant

trim it to 1 inch if it is from a flower, bigger size for corms

dipped it in bleach solution with 2 drops of surfactant (dish soap) for 20 minutes, then wash 3 times with deionized water

remove again the outter tissues since it got damaged by the bleach, now u have a perfect living sterile explant

when trimming, remember to use sharp razors or knives to avoid dulling the tissue

transfer this explant to the prepared jars contained the mixture, close the lid or by parafilm if test tubes are used.

let it sit under flourescent light for 16 hours daily at room temperature 75-85F


Most important of all, KEEP EVERYTHING CLEAN FROM BEGINNING TO END

AND REMEMBER: Not all formulas are exact, try making your variation, u dont have to be exact


and lastly

report your success with formulas back on this page

have fun growing ALL

USEFUL LINKS

: watch all of his series

this has 3 parts so find it

learn aseptic techniques from youtube

read tissue culture for home gardener there ain't error, wait 1 minutes before page load

and read anything that is on tissue culture on bananas.org


and read Ian Mcguire

LASTLY: any inputs to make the process shorten is always appreciated

Attached Images

http___www.geocities.com_k2benson_TCoverview_TCban_TCban(1).pdf (897.7 KB, 56 views)

bengtang

Here is a cached copy
Micropropagation of Musa