Thelazybanana

A cloning machine? could you further explain how it works

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Link sent me to a small business web page..nothing about bananas

Juicy Bananas

Aloha,

I have recently been allowed to use a small facility for experimenting. This man is awesome, he wants to try new things out. He has an Orchid business so my first attempt will be my Vanilla. The specimen seems to be disease free. My other thought was native bananas... maybe Ma'oli to start with, or possibly just a Mysore. My main goal to to successfully TC the Fe'i. We plan on keeping our mushrooms in his lab for safe keeping as well.

I have a lot of learning and experimenting to do... but there is a lot of information out in the world, so it will just take time.



Mahalo

chong

Quote:
Originally Posted by chong View Post
Here's one from one of our members, kgbenson:
Micropropagation of Musa

When I posted that link, it went to kgbenson's website and had a full presentation of banana tissue culture, including the selection and preparation of the meristem from the corm through various stages of division. I will try to find a copy of his presentation from that link. If I can find it, I will attach it if the copy is in word or pdf format.

Chong

greenfumb

Do you have to use a Musa pup for the donor TC. Can a young clone plant be used. I am asking because I want to try TC and I dont have any adult plants, or pups. I only have some growing clones for tissue.

Gabe15

If you are just experimenting, it is fine to use a young TC plant instead of a field grown sucker (if that's what you mean), just as long as it is a healthy growing plant.

If it were a serious professional project and you were producing them in high quantity, then it should be avoided. This is because there tends to be a small number of plants produced from TC which are "off-types", and the resulting plants are not true-to-type. If you use a young tissue cultured plant as starting stock, you have no guarantee the plant is true-to-type and you could be creating a bunch of off-type plants. For this reason, a well run banana tissue culture lab will only produce about 5 subculture cycles from a single sucker, and then discontinue that line and start over from a new field-grown sucker which has been verified true-to-type.

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druss

With regards to using the adult corm for tc, how long does the meristem last for after you cut it from the corm? Is it hrs? Days, can you preserve it for later use?

Gabe15

There would be no reason to do this and really no way to. When you excise the tissue piece containing meristem, it needs to be put into culture immediately. You would only do this in a flow hood with everything set up and ready to go. There is maybe 5-10min of prep outside the hood, and then another 5-10min in the hood for initiation, but there should be no waiting on anything as this is the most critical time to avoid contamination and to get it right. If the initial culture is contaminated then nothing can be propagated from it. If you want to preserve meristem essentially, you either leave the plant intact and growing normally, or you put it into tissue culture, for all practical purposes there is no in-between.

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Growing bananas in Colorado, Washington, Hawaii since 2004. Commercial banana farmer, 200+ varieties.

Kegas76

I think he might have been confused by the line, "For this reason, a well run banana tissue culture lab will only produce about 5 subculture cycles from a single sucker, and then discontinue that line and start over from a new field-grown sucker which has been verified true-to-type."

For those of us that have no experience with doing tissue cultures the "5 subculture cycles" sounds like 5 different batches of TCs (made from one plant) one after the other. And from that we lay-folk might assume there is a time gap between the batches.

Obviously after your last post we know this isn't the case.

druss

Sweet, i was curious if you could store meristem for future use. But makes sense when you put it that way. Just asked about some ney poovan from the tc lab where I get my plants. They told me its is one of the more difficult varieties to tc so they only keep a few plantlets.
Which is why I wondered if the meristem could be stored.

Tytaylor77

Chini chompa/ney poovan/new poovan/new pavan is an amazing grower. Great tasting tiny little bananas. Flesh is very white and harder than most. If you don't like mushy or slimey bananas you will love it. PM me and I can show some pictures.

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greenfumb

That all makes a lot of sense. I am just experimenting trying to figure out if I want to get into TC, possibly as a business in the future. But for now I am just experimenting. I have a lot of lab glassware and lab equipment from when I had a lab 10 years back. Is there a publication dealing with the chemicals/hormones banana's need during the TC propagation process.

Dalmatiansoap

One general question about TC, I'm looking for TC laboratories preferred in Europe that produce TC Olive plantlets?
Any ideas?
Thanks

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arjunasam

Hi All ,I
do lot of Tissue culture propagation in our lab Sri Lanka . Hope I can help nay body need help
It is my pleasure
regards
Arjuna

Dalmatiansoap

What kind of plants you produce in your laboratory Arjuna

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arjunasam

Hi ,
We mainly do Cavendish Banana plants for growers who do exports and some local varieties for local farmers .

arjunasam

Hi ,
We mainly do Cavendish Banana plants for growers who do exports and some local varieties for local farmers .
Other than banana , we produce lot of Ornamental plants as contract propagator for other nurseries

Stream85

thanks